Skip to content

Overnight Retinol Serum Usage Instructions

Patch Test

Before using the Overnight Retinol Serum on your face, apply a small amount on a discreet area (e.g., behind your ear or jawline) for 24-48 hours to check for any irritation.
Note: You cannot use the Overnight Retinol Serum if you’re pregnant or breastfeeding. 

How Often to Apply

For the best results, use every night. If there is any irritation, reduce the number of nights you use it per week.

How to Apply

Apply at night:
1. Cleanse your face with a gentle, non-active cleanser
2. Allow your skin to fully dry before applying
3. Apply 2–3 pumps to your face and spread a thin, even layer, avoiding the eye area, corners of the mouth, and around the nostrils
4. Wait 10–20 minutes before applying a hydrating moisturizer
5. In the morning, apply SPF 30 or higher since retinol can increase sun sensitivity

Allowed vs Not Allowed Skincare Products During Study

Allowed Products

Not Allowed Products

Gentle, non-active cleanser

Any product containing retinol, retinal, or retinoids

Basic moisturizer (no actives)

Vitamin C or brightening serums

Broad-spectrum sunscreen (SPF 30+)

AHAs/BHAs (glycolic acid, lactic acid, salicylic acid)

Hydrating toner or mist (non-exfoliating)

Peptides, growth factors, serums or creams

Eye cream (non-active)

Anti-aging serums or creams

Lip balm or facial oil (no essential oils or actives)

High-strength niacinamide (above 5%)

Sheet masks (hydrating only)

Spot treatments with acids or actives

Makeup (optional, must be removed before photos)

Exfoliating scrubs or physical exfoliants


In-office treatments (chemical peels, microneedling, lasers)


Facial tools (LED masks, microcurrent, dermaplaning, etc.)


New products (other than those provided in the study)

 

Remove this item?

Are you sure you want to remove